ABSTRACT
Objective To investigate the effect of irinotecan hydrochloride combined with cisplatin on B-cell lymphoma-2 (Bcl-2) and metastasis-associated in colon cancer-1 (MACC1) protein expressions in cervical epithelial tissue of cervical cancer patients.Methods 100 patients with cervical cancer from January 2010 to January 2013 in Department of Obstetrics and Gynecology of Shangrao City People's Hospital were selected as objects.All patients were treated with irinotecan hydrochloride and cisplatin for combination chemotherapy.The clinical effect and the protein expressions of Bcl-2 and MACC1 of cervical epithelial tissue were compared pre-and post-chemotherapy.Results The total efficiency was 75.0%(75/100) after cervical cancer patients were given irinotecan hydrochloride combined with cisplatin chemotherapy program.The expressions and high expression rates of Bcl-2 and MACC1 post-chemotherapy were significantly lower than that pre-chemotherapy(P<0.05) .In clinically effective group, the high expression rates of Bcl-2 and MACC1 post-chemotherapy significantly reduced than pre-treatment (P<0.05), and in clinical invalid group, the difference of the high expression rates of Bcl-2 and MACC1 had no statistically significant.Conclusion After combination chemotherapy with irinotecan hydrochloride and cisplatin on cervical cancer, the expression of Bcl-2 and MACC1 in cervical epithelial tissue significantly reduce, and the clinical efficacy is aignificant.
ABSTRACT
Secondary development of Chinese Patent Medicine (CPM) is an effective and innovation-driven way for the leaping development of Chinese medicine industry with less investment and faster return. Aim to improving the efficacy, safety and batch-to-batch consistency of CPMs, the theory and methodology for the secondary development of CPMs, mode for cultivating superior CPM, approaches to reforming the pharmaceutical technology and the corresponding core technologies were proposed in this paper, which is summarized as 'One objective, Three analyses, Five definitudes and Seven improvements'.
Subject(s)
Drugs, Chinese Herbal , Chemistry , Economics , Patents as Topic , Technology, Pharmaceutical , Economics , Methods , Translational Research, Biomedical , Economics , MethodsABSTRACT
This study was aimed to explore the effects of decitabine on the biological behaviour of U266 cells in vitro so as to provide a new thinking and experiment basis, as well as new evidences for the pathogenesis of multiple myeloma. MTT and colony formation assays were used to evaluate the impact of decitabine on the ability of proliferation of U266 cells; flow cytometry was used to analyze the cell distribution in cell cycle; transwell chamber and matrigel assays were used to observe the ability of migration and invasion. The results indicated that decitabine could significantly suppress the proliferation of U266 cells in time-and dose-dependent manners. The flow cytometric analysis demonstrated that the cells in G(0)-G(1) phase significantly increased while the cells in S and G(2)/M phase decreased. The migration and matrigel invading tests showed that the number of cells moving into under chamber of transwell decreased after U266 cells treated with decitabine. It is concluded that decitabine may act as an effective drug for MM by inhibiting the proliferation, migration and invasion ability, and the specific mechanism needs to be deeply explored.